目的建立银杏叶药材的快速液相(UPLC)指纹图谱，并同时测定其中11种黄酮苷类成分的含量，从而对其进行全面的质量控制，而且大大缩短了检测时限。方法采用AgilentZORBAXEclipsePlusC₁₈(4。6mm×50mm，1。8μm)色谱柱，流动相为乙腈-0。4%磷酸水溶液系统，进行梯度洗脱，流速0。6mL·min^-1，检测波长为360nm，用国家药典委员会中药色谱指纹图谱相似度评价系统软件(2004A版)处理分析，建立银杏叶药材UPLC指纹图谱。结果指纹图谱中标定了21个共有峰，以1、4、7(S)、9、10、11、12、13、14、15、16号峰为标记，以银杏叶对照药材为对照，经指纹图谱软件计算相似度，分析的32批银杏叶药材样品与对照药材之间相似度均在0。84以上，表明有一定差异；11种黄酮苷类成分在含量测定条件下，分离度高，在本实验中所述进样量范围内线性良好，加样回收率在95%~105%。结论本实验建立的方法简单，结果准确可靠，灵敏度高，重复性好，专属性强，通过测定其中黄酮苷类原型化合物的含量，能够较为精确全面地反映银杏叶药材的内在质量，结合UPLC指纹图谱为银杏叶药材的质量控制研究提供科学依据。

OBJECTIVE To establish a UPLC fingerprint of Ginkgo biloba leaves and determine the contents of eleven flavonoid glycosides. METHODS A UPLC method was established for the first time to simultaneously quantify the eleven major flavonoid glycosides in Ginkgo biloba leaves. The analysis was performed on an Agilent ZORBAX Eclipse Plus C₁₈ column(4.6 mm×50 mm, 1.8 μm), the mobile phase A was acetonitrile, the mobile phase B was 0.4% phosphoric acid, gradient elution was performed at the flow rate of 0.6 mL·min^-1, and the detection was carried out at 360 nm. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004 A) was used in data analysis. RESULTS The UPLC fingerprint of Ginkgo biloba leaves was established. Total 21 peaks were selected as the characteristic common peaks and 11 of them were identified. There were some differences in the fingerprint chromatograms between 32 batches of Ginkgo biloba leaf samples. The validation result showed that the 11 flavonoid glycosides had good linearity and recoveries. CONCLUSION The established UPLC method is simple, rapid, accurate, reliable, and sensitive, and can be applied to the identification and quality control of Ginkgo biloba leaves.